Figure 4.

Sustained GFP expression in CCE cells during differentiation into mesoderm is obtained using the CAG promoter. CCE cells were transfected with pCAGIPuro-GFP (A, B) or pCAGGSneo-GFP (C, D) and selected for puromycin or G418 resistance, respectively. FACS analysis was performed on undifferentiated CCE cells (A, C) and cells differentiated to the Flk1 stage (B, D)14–18 days posts transfection and GFP expression was compared in vectors expressing GFP (red) and empty vector (blue) transfected controls.