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. 2008 Jan 23;9:11. doi: 10.1186/1471-2199-9-11

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Copyright © 2008 Li et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Wnt3a stimulation of STF mediated by Fzd2 and Ror2 is inhibited by GSK3. H441 cells were transfected with pSV-β-gal (Promega, WI), STF, Fzd2 and Ror2WT plus Xgsk3 expression construct, and then treated with L-CM or Wnt3aCM. Equal amounts of DNA and conditioned media were used in each assay. Luciferase values were normalized to beta-galactosidase for transfection efficiency. Values of the assay transfected with control vector and treated with L-CM alone (empty bars) were adjusted to unity to normalize all other experimental values in the same group. * indicates p < 0.05.