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. Author manuscript; available in PMC: 2008 Feb 26.
Published in final edited form as: J Biol Chem. 2007 May 14;282(29):21005–21014. doi: 10.1074/jbc.M702871200

Figure 1. DGAT2 expression is strongly induced during adipocyte differentiation.

Figure 1

A, 3T3-L1 preadipocytes were induced to differentiate for various times up to 6 days (D6), RNA was isolated and DGAT1 (white bars) or DGAT2 (black bars) expression was determined by real-time PCR. Data shown are normalised to cyclophilin +/− SEM, n=4. B, DGAT2 expression was determined in RNA isolated from confluent isolated murine preadipocytes before or after induction of differentiation for various times between 4 hours (4h) and 8 days (D8). Data shown are normalised to cyclophilin +/− SD from 2 independent experiments performed with cells isolated and pooled from the adipose tissue of 4 mice in each case. C, confluent cultures of human isolated preadipocytes were induced to differentiate for various times, RNA isolated, reverse transcribed and DGAT2 expression determined by real-time PCR. Data shown are normalised to cyclophilin +/− SEM, n=5. In all cases * indicates statistically significant difference from expression at day 0 (p<0.05).