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Specificity of antisera against subunits of type II topoisomerases. Purified B. subtilis GyrA, ParC, ParE, and GyrB (0.75 μg each) were analyzed on an SDS gel. The gel either was stained for total protein or was processed for immunoblotting. (A) Stained with SYPRO Red (Molecular Dynamics). (B) Probed with antiserum against ParC (residues 499–806). (C) Probed with antiserum against GyrA (residues 1–607). (D) Probed with antiserum against full length ParE.
