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. Author manuscript; available in PMC: 2008 Oct 1.
Published in final edited form as: Nat Cell Biol. 2007 Sep 23;9(10):1142–1151. doi: 10.1038/ncb1634

Figure 4.

Figure 4

Bif-1 localizes to autophagosomes. (a) COS7 cells were transfected with Bif-1-HcRed together with GFP-Atg5 or GFP-LC3. Twenty hours after transfection, the cells were cultured in EBSS or CM for 2 h and subjected to confocal microscopic analysis. Magnified images are shown as insets. Representative Bif-1-Atg5 or Bif-1-LC-3 double positive foci are indicated by arrows. Scale bars in the insets are 1 μm. (b) COS7 cells transfected with Bif-1-GFP were cultured in EBSS for 2 h and the localization of Bif-1-GFP was examined by immunogold electron microscopy using anti-GFP antibody. Arrows indicate representative gold particles that are detected on autophagosomal membranes.