Figure 2.

PCR in situ (direct and indirect) of normal human trigeminal ganglia with VZV gene primers. (a) Sample 16 amplified with primers to VZV gene 18 (direct PCR in situ). (b and c) Sample 12 and sample 19 amplified with primers to VZV gene 29 (direct PCR in situ). (d) Sample 12 amplified with primers to VZV gene 29 and the section subsequently hybridized to the DIG-labeled internal 70-mer oligonucleotide probe (indirect PCR in situ). Positive signals in neurons can be seen in all panels. (×450.)