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Nature of the enlarged compartment in MCOLN1 RNAi clones. Confocal images of RAW264.7 and LS9 cells whose terminal compartments were pre-loaded with Dextran-Rhodamine (red) and stained for the indicated markers (green). All of the makers were detected using immuno-specific antibodies, except for GFP-Rab7 that was transfected into wild type and MCOLN1- cells. Bar is 5 μm.
