Figure 1.

Dual color FISH with two different plasmids detecting the same transgene array. This 50 Mbp transgene array is composed of multiple transgene copies as well as host DNA. pPALZ8.8 (green and center, labeled with FITC) was originally used to generate the transgene array and thus completely covers transgenes while pPS8.8 (red, bottom, labeled with Cy5) detects only the lacO sequence which comprises one sixth of the complete transgene length. CC values are given in percent. They were calculated in 3D, thus they do not necessarily reflect similarity in the projections of deconvolved image stacks shown here. Scale bar: 2 μm.