Table 1.
Function of candidate reference genes. Amplification efficiency is determined using the formula 10-1/slope. For the actual calculations, the base of the exponential amplification function is used (e.g. 1.96 means 96% amplification efficiency).
| Gene symbol | Sequence name | Function | Primerconc. (nM) | Amplification efficiency |
| act-1 | T04C12.6 | Cytoskeletal structural protein | 200 | 1.96 |
| ama-1 | F36A4.7 | RNA polymerase II | 400 | 1.85 |
| cdc-42 | R07G3.1 | RHO GTPase | 200 | 1.95 |
| csq-1 | F40E10.3 | Calcium ion binding protein | 200 | 1.95 |
| eif-3.C | T23D8.4 | Translation initiation factor | 300 | 1.92 |
| mdh-1 | F20H11.3 | Malate dehydrogenase | 300 | 1.93 |
| gpd-2 | K10B3.8 | Glyceraldehyde-3-phosphate dehydrogenase | 300 | 1.94 |
| pmp-3 | C54G10.3 | Acyl-CoA transporter | 300 | 1.89 |
| tba-1 | F26E4.8 | Alpha tubulin | 200 | 1.95 |
| Y45F10D.4 | Y45F10D.4 | Iron binding protein involved in Fe-S cluster formation | 200 | 2.07 |