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Alkaline phosphatase activity in external membrane fragments compared to whole BeWo cells, and inhibition of ALP activity by levamisole. BeWo cells or cell membrane fractions were preincubated with 500 μM levamisole followed by a 60-minute reaction of 1 mM 4-nitrophenylphosphate conversion to 4-nitrophenol. Data represent the average and standard deviation of 4 determinations, corrected for protein content. * Significantly different from control (p < 0.05).
