Table 1.

Relative distribution of synapsins in subcellular fractions

	Antibody	Fold enrichment in synaptic vesicles	Depletion after salt incubation, %
Synapsin Ia	G304	13.1 ± 1.5	88 ± 5
Synapsin IIa	G304	11.6 ± 0.5	70 ± 2
Synapsin IIIa	G304	11.2 ± 1.0	78 ± 1
Synaptophysin	G95	14.8	<1

Immunoblotting of purified synaptic vesicles was carried out as described in Fig. 5 with the indicated antibodies. Enrichment values refer to the fold enrichment in synapsin immunoreactivity with respect to forebrain homogenate. Synapsins were depleted from purified synaptic vesicles by incubating the vesicles, diluted to 10 μg/ml, in the presence of 200 mM NaCl for 2 hr at 0°C. Data are the mean ± SEM (n = 3) for synapsins and a single experiment for synaptophysin.