Table 1.
Summary of coimmunoprecipitations
| Protein | IP with Src Ab |
|---|---|
| Dynamin | + |
| Snapsins Ia and Ib | + |
| Synaptophysin | − |
| Synaptotagmin | − |
| Syntaxin | − |
| Rab3a | − |
| Transferrin receptor | − |
| Grb2 | − |
| Phospholipase C-γ | − |
| α-Adaptin | + |
| γ-Adaptin | − |
| Clathrin | − |
PC12 cells were lysed and prepared for immunoprecipitation (IP) with normal rabbit serum or Src antibody. Whole cell lysate and the immunoprecipitates were Western blotted with the indicated antibodies. A + indicates that the antigen was detected in the immunoprecipitate of Src but not normal rabbit serum when 10-fold more protein than required to detect the antigen by Western blotting was used. A − indicates that the antigen was not detected.