Table 1.
Trafficking-Defective Mutants
| Loop | Mutations | Domaina | Replicationb | Traffickingc |
|---|---|---|---|---|
| Wild type | 100% | 11/12 | ||
| 6 | G36U/A37C/C38G | TL | 30% | 0/12 |
| 10 | A290Δ/A291Δ | P | 32% | 0/12 |
| 12 | A77Δ/G78Δ/C79Δ | CR | 29% | 0/12 |
| 17 | A118G/A119C/A120Δ | CR | 52% | 0/12 |
| 18 | A126G/C127Δ | V | 63% | 0/12 |
| 19 | C227U | V | 46% | 0/12 |
| 20 | A222G/C223Δ | V | 73% | 0/12 |
| 24 | U157G/A158G/U161A | TR | 25% | 0/12 |
| 25 | C166A/C167A | TR | 29% | 0/12 |
| 26 | C172GGAd | TR | 46% | 0/12 |
a
Structural domains in native secondary structure. TL, left terminal; P, pathogenicity; CR, central region; V, variable; TR, right terminal.
b
Replication efficiency of mutants expressed as a percentage of wild-type PSTVd.
c
Trafficking function is expressed as number of plants showing systemic infection, determined by dot blot, over total number of plants inoculated.
d
Insertion of GA between residues 172 and 173.