Table 4.
Mutation | Metastable Structure | Domain | Replication | Trafficking | Genetic Stability | Tomato Infectivity |
---|---|---|---|---|---|---|
Wild type | 100% | 11/12 | S | S | ||
G26C | TL | 23% | 0/12 | N1 | ||
G46C | TL | 42% | 0/12 | N1 | ||
G65U | P | 46% | 1/12 | R | N2 | |
C229U | HPII-Dis | V | 54% | 3/12 | R/M | R3 |
C231A | HPII-Dis | V | 95% | 9/12 | S/R/M | N3 |
G324U | HPII-Dis | TL | 82% | 8/12 | S/R/M | N3 |
C231A/G324U | HPII-Res | TL+V | 86% | 8/12 | S | N3 |
G80A | HPI-Dis | CR | 53% | 0/12 | N1 | |
C109U | HPI-Dis | CR | 72% | 0/12 | N1 | |
G80A/C109U | HPI-Res | CR | 66% | 0/12 | N1 |
See Table 1 for legend. S, stable; R, reversion to the wild type; M, additional mutations; N, noninfectious; Dis, predicted to disrupt HPI or HPII structure; Res, predicted to restore HPI or HPII structure. 1, Hammond and Owens (1987); 2, Owens et al. (1991); 3, Qu et al. (1993).