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. 2008 Jan;20(1):48–58. doi: 10.1105/tpc.107.056127

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Copyright © 2008, American Society of Plant Biologists

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Figure 5. — Related Sequence Motifs Mediate AS1-AS2 Binding to BP and KNAT2.

(A) to (D) The AS1 complex binds to two sites in the KNAT2 promoter. The diagram of the KNAT2 promoter indicates the relative positions of four of the 14 promoter fragments analyzed by ChIP. Numbers indicate distance in base pairs from the translation initiation site. Lanes 1 to 3, ChIP on wild-type seedlings; lanes 4 to 6, ChIP on AS1_pro >AS1-HA transgenic seedlings; lanes 1 and 4, total DNA; lanes 2 and 5, mock ChIP; lanes 3 and 6, ChIP with HA antibodies.

(A) and (B) ChIP on wild-type and AS1_pro >AS1-HA seedlings shows association of the AS1 complex with KNAT2 promoter fragments A and B.

(C) and (D) ChIP results for two promoter fragments that do not interact with the AS1 complex.

(E) Sequence motifs related to the AS1-AS2 cis-regulatory elements in BP are present in the AS1 complex binding sites A and B of the KNAT2 promoter. Inferred consensus sequences for the AS1 and AS2 binding motifs and their positions relative to the start codon of BP or KNAT2 are also shown.