Figure 2.

Functional Analysis of GNOM Mutant Proteins.

(A) to (F) Interallelic complementation of endogenous (G) and transgenic (T) GNOM variants: schematic showing heterologous dimerization module XLIM and XLDB, Myc or HA tag, and amino acid exchange mutations (left panels). G1, genomic allele 1; G2, genomic allele 2; T1, transgene 1; T2, transgene 2. Phenotypes of homozygous gnom mutant seedlings harboring the respective GNOM transgene(s) (center panels). Protein gel blot analysis of transgenic expression (right panels).

(A) to (C) Complementation. Bars = 3 mm.

(D) to (F) No complementation. Bars = 0.1 mm.