Figure 8.

Incorporation of ³⁵SO₄²⁻ into Cellular Sulfur Compounds in Vivo.

Leaf pieces of the wild type and the four indicated oastl mutant genotypes were incubated with ³⁵SO₄²⁻-spiked half-strength Hoagland solution for 15 min and subsequently kept on the same medium without radiolabel until 45 min after the start of the experiment. Samples were taken at the indicated time points. Sulfate, thiols, and proteins were separated by HPLC or specific precipitation, and incorporated ³⁵S was quantified by scintillation counting. FW, fresh weight. Mean values ± sd are shown. * P < 0.05 > 0.01; ** P < 0.01 > 0.001.