Table 1.
Purification of OAS-TL Proteins from Wild-Type Leaf Extracts in Extraction Buffer B Using SAT Affinity Chromatography
| Fraction | Total Protein (mg) | Total Activity (μmol/min) | Specific Activity (μmol·min−1·mg−1) | Yield (%) | Purification Level |
|---|---|---|---|---|---|
| Crude extract | 257 | 488 ± 77 | 1.9 ± 0.3 | 100 | 1 |
| Flowthrough | 245 | 44 ± 3 | 0.18 ± 0.01 | 9 | 0.09 |
| Elution | 0.58 | 183 ± 19 | 315 ± 32 | 38 | 166 |