Figure 3.

Complementation of Yeast mms2 Mutants by At UEV1.

(A) Complementation of the mms2 single mutant by At UEV1. WXY942 (mms2Δ) transformants were grown overnight and printed onto YPD and YPD + 0.025% MMS gradient plates. The plates were incubated at 30°C for 2 d before being photographed. The arrow indicates higher MMS concentrations. Several transformants of each treatment were tested with the same result, and only one is shown here.

(B) Complementation of the mms2 ubc13 double mutant (WXY955) by At UEV1A, At UEV1D, and At UBC13. Experimental conditions were as in (A).