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PCR analysis of rabbit brain and heart RNA for presence of β2a (β2a0B) and β2d (B2a-C) sequences. Top, diagram of β subunit sequence homology domains and relative location of forward (a, c, and e) and reverse (b, d, and f) primers. a and b primers test for presence of β2 sequences regardless of composition of the variable D1 or D5 domians; primers c and d and primers e and f test for presence of sequences encoding the cardiac β2a (β2a-C) or brain β2a (β2a-B) N-termini.
