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Q-PCR validation of microarray data. Fold difference of RNA levels of genes identified in the microarray analysis (grey bars) were analyzed in 104-C1-GFP and 104-C1-GFPOrfA cells by Q-PCR (white bars). (A) RNA fold differences for four genes identified as highly regulated by OrfA. (B) RNA fold differences for five regulated genes of the splice factor and proteasome families.
