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. 1998 Apr 14;95(8):4766–4771. doi: 10.1073/pnas.95.8.4766

Figure 3.

Figure 3

Expression of ACS5 mRNA in wild-type and mutant lines. (A) Time course of ACS5 mRNA accumulation. Intact 3-day-old etiolated seedlings were incubated in 5 μM benzyladenine for the times indicated; total RNA was isolated and analyzed by Northern blotting. The blot was hybridized with an ACS5 probe, stripped, and rehybridized with a β-tubulin probe as a loading control. Hybridization intensities were quantified with a PhosphorImager (Molecular Dynamics). The level of ACS5 expression was normalized to that of β-tubulin and plotted. (Inset) The original images of the blot. (B) Northern analysis of RNA from variously treated seedlings. Poly(A)+ RNA was prepared from 3-day-old etiolated seedlings grown as follows: lane 1, acs5-2 plants grown on MS medium; lane 2, acs5-1 plants grown on MS medium or lane 3, MS medium plus 5 μM benzyladenine; lane 4, wild-type (Col) grown on MS medium; and lane 5, eto2 grown on MS medium. Note that lanes 4 and 5 are from an independent experiment.