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Formation of a–c cross-links by CuP reaction at 10°C in a series of doubly Cys-substituted membranes. The conditions are as described in Fig. 3. The positions of the double Cys substitutions are indicated. Wild-type membranes are from a pDF163 transformant of strain JWP109 (ΔuncB–H). Deletion membranes are from strain JWP109 (ΔuncB–H).
