Table 1.
Effect of temperature on the yield of a–c cross-links
| Position of Cys substituions |
a in cross-link, %
|
||
|---|---|---|---|
| 0°C | 10°C | 20°C | |
| a207/c54 | 7 ± 1 | 10 ± 3 | 19 ± 2 |
| a207/c55 | 11 ± 2 | 12 ± 3 | 16 ± 4 |
| a207/c62 | 3 ± 1 | 5 ± 2 | 15 ± 4 |
| a214/c58 | 0 | 3 ± 1 | 10 ± 3 |
| a214/c62 | 5 ± 0 | 21 ± 4 | 29 ± 4 |
| a214/c65 | 6 ± 0 | 25 ± 4 | 36 ± 2 |
| a214/c66 | 0 | 7 ± 3 | 16 ± 3 |
| a214/c69 | 0 | 3 ± 2 | 10 ± 1 |
| a221/c65 | 0 | 6 ± 1 | 26 ± 6 |
| a221/c66 | 0 | 1 ± 1 | 17 ± 4 |
| a221/c69 | 5 ± 1 | 18 ± 3 | 40 ± 4 |
| a223/c72 | 12 ± 0 | 32 ± 3 | 42 ± 3 |
| a224/c72 | 2 ± 1 | 9 ± 3 | 27 ± 7 |
| a224/c73 | 58 ± 0 | 65 ± 0 | 81 ± 8 |
| a225/c72 | 0 | 6 ± 2 | 11 ± 5 |
| a225/c73 | 3 ± 0 | 24 ± 2 | 35 ± 7 |
All mutant pairs forming ≥10% a–c cross-links at 20°C are shown. The percentage of immunopositive subunit a in the a–c cross-link is indicated (average ± SD of two to four experiments for each pair).