Table 1.
Enzymatic activity and stability of the bifunctional insertion fusion protein GluXyn-1 compared with 1,3-1,4-β-glucanase of B. macerans and 1,4-β-xylanase of B. subtilis
| GluXyn-1 (1,3-1,4-β-glucanase activity) | wtGLU | GluXyn-1 (1,4-β-xylanase activity) | wtXYN | |
|---|---|---|---|---|
| Km, mg/ml | 0.87 ± 0.01 | 0.94 ± 0.07 | 3.36 ± 0.25 | 3.17 ± 0.25 |
| kcat, s−1 | 1,012 ± 1 | 1,040 ± 26 | 582 ± 20 | 705 ± 25 |
| Vmax, units/mg | 1,380 ± 1 | 2,399 ± 60 | 794 ± 28 | 2,015 ± 72 |
| pH for optimal activity | 6–7.5 | 6–7.5 | 6 | 6 |
| Temperature for optimal activity, °C | 55–60 | 65–70 | 50 | 55 |
| Thermostability t1/2 (min) at 60°C, pH 5 | 135 | 100% residual activity after 240 min | 118 | 90% residual activity after 240 min |