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. Author manuscript; available in PMC: 2009 Jan 30.
Published in final edited form as: J Neurosci Methods. 2007 Sep 7;167(2):292–301. doi: 10.1016/j.jneumeth.2007.08.028

Fig. 1. Culture Paradigms.

Fig. 1

(A) Following dissection and digestion cortical neurons were prepared in either NB or NB-A3 (washes, dissociation and counting). Neurons in NB-A3 remained in this media for plating while neurons in NB media were subsequently divided and placed into either NB or NB-A25 for plating. Neurons plated in NB-A3 or NB-A25 were maintained by either standard or restorative feedings. The NB cultures were maintained by standard feedings only. (B) Following dissection and digestion cortical neurons were divided and prepared in NB-A3 or NB-A25. Following culture preparation each group was further divided into two groups: the first was plated in the same media as the preparation media maintaining a consistent glucose concentration, the second was plated in media supplemented with a different glucose concentration than was present during preparation. All cultures in this paradigm were maintained by the restorative feeding strategy.