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. 1998 Jun 9;95(12):6619–6623. doi: 10.1073/pnas.95.12.6619

Figure 4.

Figure 4

Impact of promoter choice on F5H overexpression in transgenic Arabidopsis. Stem tissue from 5-week-old plants of the wild type, the fah1–2 mutant, and nine independent fah1–2 lines homozygous for the 35S-F5H transgene (A) was harvested and used for RNA isolation. Blots were probed with the F5H cDNA and were exposed to film for 24 hr to visualize the level of F5H expression in the wild type and the fah1–2 mutant (Left), and for 2 hr to evaluate F5H expression in the 35S-F5H transgenics (Right). Identical analyses were carried out on 5-week-old stem tissue from the wild type, the fah1–2 mutant, and nine fah1–2 lines homozygous for the C4H-F5H transgene (B). Blots were probed with the F5H cDNA and were exposed to film for 12 hr to visualize the level of F5H expression.