. Author manuscript; available in PMC: 2008 Feb 27.

Published in final edited form as: J Dent Res. 2006 Oct;85(10):878–887. doi: 10.1177/154405910608501002

Table.

Biofilm Analyses and Enamel Demineralization from Previously Published Studies

		Biofilm Concentrations				Mineral Loss
Paper	Treatment	F, μg/g³	Ca, mg/g³	P_i, mg/g³	EPS, mg/g³	SMH Change (%)⁴	ΔZ⁵
Cury et al., 1997 ¹	Sucrose 0	18.7 ± 3.3^c	12.9 ± 2.5^b	3.7 ± 1.1^b	12.0 ± 2.6^b
	Sucrose 2x	9.6 ± 2.8^b	4.0 ± 1.0^a	0.8 ± 0.2^a	11.5 ± 1.8^b
	Sucrose 4x	2.7 ± 0.8^a	4.0 ± 1.1^a	0.4 ± 0.1^a	14.0 ± 3.3^b	--	--
	Sucrose 8x	2.7 ± 0.4^a	4.2 ± 1.1^a	0.4 ± 0.04^a	35.8 ± 6.9^a

Cury et al., 2000 ¹	Control	140.6 ± 30.8^a	17.0 ± 2.8^a	11.5 ± 2.1^a	6.5 ± 1.0^a	- 3.3 ± 0.7^a	^χ27,021.7 ± 3951.5^a
	Glucose 10% + Fructose 10%	27.4 ± 15.9^b	1.9 ± 0.7^b	0.5 ± 0.1^b	11.8 ± 1.9^a	-44.2 ± 7.2^b	20,159.5 ± 2213.4^b
	Sucrose 20%	5.6 ± 2.2^b	0.6 ± 0.1^b	0.3 ± 0.04^b	35.0 ± 7.8^b	-73.4 ± 6.9^c	12,414.4 ± 2419.4^c

Cury et al., 2001	Sucrose 0						^χ26,949.2 ± 632.1^a
	Sucrose 2x						25,527.6 ± 785.0^a
	Sucrose 4x	--	--	--	--		24,000.8 ± 1157.5^a
	Sucrose 8x						15,887.4 ± 2739.2^b

Nobre dos Santos et al., 2002 ²	Caries-free	58.1 ± 21.3^a	10.6 ± 5.4^a	6.0 ± 2.9^a	39.2 ± 7.4^a
	Pit/Fissure Caries	32.5 ± 13.6^b	7.9 ± 4.3^a	4.0 ± 1.9^b	47.4 ± 8.9^b
	Nursing Caries	6.2 ± 2.9^c	3.3 ± 2.6^b	2.6 ± 1.3^b	55.6 ± 17.6^b

Cury et al., 2003 ¹	Sucrose Exposure	1.1 ± 0.3^a	1.2 ± 0.7^a	0.2 ± 0.04^a,b	51.1 ± 13.6^a
	Sucrose Interruption:
	24 hrs	1.6 ± 0.4^a	1.6 ± 0.6^a	0.2 ± 0.1^a	49.7 ± 11.4^a
	48 hrs	2.7 ± 0.6^a	3.0 ± 1.5^a	0.4 ± 0.1^b	40.6 ± 9.6^b	--	--
	Sucrose Absence	63.3 ± 23.6^a	12.2 ± 1.9^a	4.3 ± 1.5^a	4.6 ± 0.5^a
	Sucrose Exposure:
	24 hrs	86.1 ± 38.6^a	18.8 ± 4.5^a	4.9 ± 1.3^a	10.0 ± 2.7^a,b
	48 hrs	67.9 ± 37.5^a	14.8 ± 4.0^a	3.9 ± 1.1^a	11.4 ± 2.3^b

Paes Leme et al., 2004b¹	Sucrose 4x	31.0 ± 41.7^a	0.73 ± 0.52^a	1.0 ± 0.9^a	29.1 ± 17.5^a	-19.7 ± 17.5^a	^δ 655.7 ± 506.2^a
	Sucrose 8x	17.3 ± 28.6^b	0.53 ± 0.37^b	0.7 ± 0.7^a	44.3 ± 25.5^b	-29.5 ± 24.6^b	^δ 869.8 ± 726.3^b

Pecharki et al., 2005 ²	Control	352.0 ± 271.0^a	45.9 ± 27.0^a	23.5 ± 14.2^a	35.4 ± 7.4^a	- 5.1 ± 7.6^a	^δ 459.4 ± 212.6^a
	20% Sucrose	28.2 ± 79.0^b	2.1 ± 1.8^b	3.0 ± 1.5^b	194.0 ± 125.0^b	-57.3 ± 32.3^b	^δ 2,165.5 ± 1890.3^b

Ribeiro et al., 2005 ²	Control	468.4 ± 401.8^a	45.9 ± 50.9^a	27.1 ± 29.5^a	47.5 ± 22.8^a		^δ 447.9 ± 169.0^a
	2% Starch	239.8 ± 251.3^b	17.4 ± 25.4^b	11.6 ± 14.6^b	49.8 ± 13.5^a		^δ 420.0 ± 160.1^a
	20% Sucrose	119.9 ± 179.3^c	5.1 ± 7.9^c	4.1 ± 4.6^c	181.6 ± 115.8^b	--	^δ 955.6 ± 543.6^b
	Starch + Sucrose	55.7 ± 144.3^c	4.9 ± 10.5^c	3.6 ± 5.0^c	201.6 ± 137.6^b		^δ 1,421.8 ± 653.8^c

Aires et al., 2006 ²	Control	55.1 ± 46.1^a	39.4 ± 17.2^a	22.0 ± 10.5^a	28.1 ± 6.5^a	- 3.9 ± 5.0^a	^δ 253.0 ± 129.0^a
	1% Sucrose	64.0 ± 82.8^a	25.1 ± 23.1^a	14.9 ± 12.2^a	41.6 ± 13.5^b	-10.5 ± 14.5^a	382.0 ± 224.0^a
	5% Sucrose	35.4 ± 53.4^ab	5.7 ± 5.3^b	4.2 ± 3.0^b	109.0 ± 85.9^c	-48.8 ± 34.4^b	796.0 ± 600.0^b
	10% Sucrose	11.9 ± 11.9^bc	4.7 ± 5.1^b	3.2 ± 2.2^b	102.0 ± 76.0^c	-43.8 ± 31.2^b	1,050.0 ± 1240.0^b
	20% Sucrose	12.3 ± 14.8^bc	5.4 ± 6.1^b	3.7 ± 2.6^b	125.0 ± 85.4^c	-58.2 ± 32.6^b	1,580.0 ± 1540.0^bc
	40% Sucrose	5.5 ± 2.4^c	2.6 ± 1.0^b	2.3 ± 0.8^b	268.0 ± 163.0^d	-65.7 ± 40.1^b	1,600.0 ± 862.0^c

Wet biofilm basis.

Dry biofilm basis.

Distinct superscript lower-case letters show statistical difference between treatments (p < 0.05).

⁴

Surface microhardness change.

⁵

% vol. min × μm.