Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
SYBR Green melting curve for quantitative real time RT-PCR. The melting curves for human lung mast cell QRT-PCR consistently gave a single peak with no evidence of non-specific amplification or primer-dimerisation. The graph is from a single experiment and representative of all donors.
