Table 2.

Comparison between Y. pestis product sizes inferred by Agilent 2100 Bioanalyzer software (OBSERVED SIZE) and actual sizes obtained by direct sequencing of the PCR product or data available in Genbank (EXPECTED SIZE). UL BPS (unit length size).

MULTIPLEX	MARKER (final primer concentration, μM)	UL BPS	ALLELE	EXPECTED SIZE	OBSERVED SIZE	OFFSET
1	ypms51 (0.2)	21	1	186	185	1
			2	207	207	0
	ypms01* (0.2)	18	6	283	279	4
			7	301	291	10
			9	337	329	8
			10	355	344	11
2	ypms04 (0.2)	17	5	179	187	-8
			6	196	205	-9
			8	230	241	-11
			9	247	259	-12
	ypms62* (0.2)	9	5	270	258	12
			6	279	267	12
			9	306	285	21
			10	315	294	21
			12	333	308	25
			15	360	331	29
			20	405	366	39
3	ypms07 (0.2)	10	7	164	168	-4
			8	174	178	-4
			9	184	187	-3
	ypms05 (0.2)	17	10	274	285	-11
			11	291	300	-9
4	ypms74 (0.2)	15	5	180	178	2
			6	195	190	5
			7	210	203	7
			8	225	217	8
	ypms15* (0.2)	15	9	275	272	3
			10	290	284	6
5	ypms35 (0.2)	15	8	204	208	-4
			9	219	222	-3
	ypms40* (0.2)	17	7	253	252	1
			8	270	266	4
			9	287	277	10
6	ypms38 (0.2)	16	6	201	200	1
			7	217	215	2
			8	233	230	3
	ypms20* (0.2)	15	8	288	285	3
			9	303	300	3
7	ypms41 (0.2)	17	5	183	188	-5
			6	200	207	-7
			7	217	221	-4
	ypms56* (0.2)	16	7	258	265	-7
			9	290	296	-6
8	ypms45 (0.2)	12	6	149	147	2
			7	161	159	2
	ypms44 (0.2)	17	7	233	239	-6
			8	250	260	-10
	ypms09* (0.2)	18	22	732	671	61
			32	930	847	83
			35	984	912	72
9	ypms71 (0.2)	14	5	157	163	-6
			6	171	178	-7
	ypms46 (0.2)	7	3	238	243	-5
			4	245	250	-5
			5	252	256	-4
			6	259	263	-4
			10	308	313	-5
			12	322	326	-4
10	ypms70 (0.2)	9	5	137	145	-8
			6	146	155	-9
			8	164	174	-10
			9	173	185	-12
			11	191	205	-14
	ypms54 (0.2)	22	6	259	265	-6
			7	281	289	-8
11	ypms69 (0.2)	16	5	163	164	-1
			6	179	177	2
			7	195	195	0
	ypms06 (0.2)	60	3	306	309	-3
			4	366	374	-8
			6	486	504	-18
			8	606	625	-19
			9	666	674	-8
12	ypms73 (0.2)	18	5	207	212	-5
			6	225	231	-6
	ypms21* (0.2)	18	9	329	339	-10
			10	347	358	-11
			11	365	375	-10

* modified primer set (increased final fragment size)