Table 2.
GlaI activity on methylated oligonucleotide duplexes
| Oligonucleotide duplex | The central part of structure of a duplex | Relative enzyme's activity (%) and standard error |
| G7*/G8 | 5'-TTTTCGMGMTGACG-3' | 100 ± 5 |
| 3'-AAAAGMGMGACTGC-5' | ||
| G8*/G7 | 5'-CGTCAGMGMGAAAA-3' | 97 ± 5 |
| 3'-GCAGTMGMGCTTTT-5' | ||
| G3*/G8 | 5'-TTTTCGMGCTGACG-3' | 28 ± 2 |
| 3'-AAAAGMGMGACTGC-5' | ||
| G8*/G3 | 5'-CGTCAGMGMGAAAA-3' | 40 ± 2 |
| 3'-GCAGTCGMGCTTTT-5' | ||
| G4*/G7 | 5'-CGTCAGMGCGAAAA-3' | 31 ± 2 |
| 3'-GCAGTMGMGCTTTT-5' | ||
| G7*/G4 | 5'-TTTTCGMGMTGACG-3' | 36 ± 2 |
| 3'-AAAAGCGMGACTGC-5' | ||
| G11*/G12 | 5'-TTTTCAMGMTGACG-3' | 61 ± 3 |
| 3'-AAAAGTGMGACTGC-5' | ||
| G12*/G11 | 5'-CGTCAGMGTGAAAA-3' | 60 ± 3 |
| 3'-GCAGTMGMACTTTT-5' | ||
| G6*/G7 | 5'-CGTCAGCGMGAAAA-3' | 16 ± 1 |
| 3'-GCAGTMGMGCTTTT-5' | ||
| G7*/G6 | 5'-TTTTCGMGMTGACG-3' | 17 ± 1 |
| 3'-AAAAGMGCGACTGC-5' | ||
| G5*/G8 | 5'-TTTTCGCGMTGACG-3' | 17 ± 1 |
| 3'-AAAAGMGMGACTGC-5' | ||
| G8*/G5 | 5'-CGTCAGMGMGAAAA-3' | 17 ± 1 |
| 3'-GCAGTMGCGCTTTT-5' | ||
| G13*/G14 | 5'-TTTTCGTGMTGACG-3' | 21 ± 1 |
| 3'-AAAAGMAMGACTGC-5' | ||
| G14*/G13 | 5'-CGTCAGMAMGAAAA-3' | 16 ± 1 |
| 3'-GCAGTMGTGCTTTT-5' | ||
| G20*/G21 | 5'-TTTTCGMAMTGACG-3' | 17 ± 1 |
| 3'-AAAAGMGTGACTGC-5' | ||
| G21*/G20 | 5'-CGTCAGTGMGAAAA-3' | 25 ± 1 |
| 3'-GCAGTMAMGCTTTT-5' | ||
| G3*/G4 | 5'-TTTTCGMGCTGACG-3' | 5 ± 1 |
| 3'-AAAAGCGMGACTGC-5' | ||
| G4*/G3 | 5'-CGTCAGMGCGAAAA-3' | 6 ± 1 |
| 3'-GCAGTCGMGCTTTT-5' | ||
| G12*/G17 | 5'-CGTCAGMGTGAAAA-3' | 28 ± 1 |
| 3'-GCAGTCGMACTTTT-5' | ||
| G17*/G12 | 5'-TTTTCAMGCTGACG-3' | 13 ± 1 |
| 3'-AAAAGTGMGACTGC-5' | ||
| G15*/G16 | 5'-TTTTCAMGTTGACG-3' | 26 ± 1 |
| 3'-AAAAGTGMAACTGC-5' | ||
| G16*/G15 | 5'-CGTCAAMGTGAAAA-3' | 23 ± 1 |
| 3'-GCAGTTGMACTTTT-5' | ||
| G18*/G19 | 5'-TTTTCGMGTTGACG-3' | 12 ± 1 |
| 3'-AAAAGCGMAACTGC-5' | ||
| G19*/G18 | 5'-CGTCAAMGCGAAAA-3' | 14 ± 1 |
| 3'-GCAGTTGMGCTTTT-5' | ||
| G7*/G2 | 5'-TTTTCGMGMTGACG-3' | < 1 |
| 3'-AAAAGCGCGACTGC-5' | ||
| G2*/G7 | 5'-CGTCAGCGCGAAAA-3' | < 1 |
| 3'-GCAGTMGMGCTTTT-5' | ||
| G13*/G35 | 5'-TTTTCGTGMTGACG-3' | < 1 |
| 3'-AAAAGMACGACTGC-5' | ||
| G35*/G13 | 5'-CGTCAGCAMGAAAA-3' | < 1 |
| 3'-GCAGTMGTGCTTTT-5' | ||
| G5*/G6 | 5'-TTTTCGCGMTGACG-3' | < 1 |
| 3'-AAAAGMGCGACTGC-5' | ||
| G6*/G5 | 5'-CGTCAGCGMGAAAA-3' | < 1 |
| 3'-GCAGTMGCGCTTTT-5' | ||
| G12*/G32 | 5'-CGTCAGMGTGAAAA-3' | < 1 |
| 3'-GCAGTMGCACTTTT-5' | ||
| G32*/G12 | 5'-TTTTCACGMTGACG-3' | < 1 |
| 3'-AAAAGTGMGACTGC-5' | ||
| G33*/G34 | 5'-TTTTCATGMTGACG-3' | < 1 |
| 3'-AAAAGTAMGACTGC-5' | ||
| G34*/G3 | 5'-CGTCAGMATGAAAA-3' | < 1 |
| 3'-GCAGTMGTACTTTT-5' | ||
| G13*/36 | 5'-TTTTCGTGMTGACG-3' | < 1 |
| 3'-AAAAGCAMGACTGC-5' | ||
| G36*/13 | 5'-CGTCAGMACGAAAA-3' | < 1 |
| 3'-GCAGTMGTGCTTTT-5' | ||
| G3*/G6 | 5'-TTTTCGMGCTGACG-3' | < 1 |
| 3'-AAAAGMGCGACTGC-5' | ||
| G6*/G3 | 5'-CGTCAGCGMGAAAA-3' | < 1 |
| 3'-GCAGTCGMGCTTTT-5' | ||
| G4*/G5 | 5'-CGTCAGMGCGAAAA-3' | < 1 |
| 3'-GCAGTMGCGCTTTT-5' | ||
| G5*/G4 | 5'-TTTTCGCGMTGACG-3' | < 1 |
| 3'-AAAAGCGMGACTGC-5' | ||
| G22*/G23 | 5'-TTTTCMMGMTGACG-3' | < 1 |
| 3'-AAAAGGGMGACTGC-5' | ||
| G23*/G22 | 5'-CGTCAGMGGGAAAA-3' | < 1 |
| 3'-GCAGTMGMMCTTTT-5' | ||
| G24*/G25 | 5'-TTTTCGGGMTGACG-3' | < 1 |
| 3'-AAAAGMMMGACTGC-5' | ||
| G25*/G24 | 5'-CGTCAGMMMGAAAA-3' | < 1 |
| 3'-GCAGTMGGGCTTTT-5' | ||
| G28*/G29 | 5'-TTTTCTMGMTGACG-3' | < 1 |
| 3'-AAAAGAGMGACTGC-5' | ||
| G29*/G28 | 5'-CGTCAGMGAGAAAA-3' | < 1 |
| 3'-GCAGTMGMTCTTTT-5' | ||
| G30*/G31 | 5'-TTTTCGAGMTGACG-3' | < 1 |
| 3'-AAAAGMTMGACTGC-5' | ||
| G31*/G30 | 5'-CGTCAGMTMGAAAA-3' | < 1 |
| 3'-GCAGTMGAGCTTTT-5' | ||
| G37*/G38 | 5'-TTTTCTMGATGACG-3' | < 1 |
| 3'-AAAAGAGMTACTGC-5' | ||
| G38*/G37 | 5'-CGTCATMGAGAAAA-3' | < 1 |
| 3'-GCAGTAGMTCTTTT-5' | ||
| G26*/G23 | 5'-TTTTCCMGMTGACG-3' | < 1 |
| 3'-AAAAGGGMGACTGC-5' | ||
| G23*/G26 | 5'-CGTCAGMGGGAAAA-3' | < 1 |
| 3'-GCAGTMGMCCTTTT-5' | ||
| G27*/G24 | 5'-CGTCAGMCMGAAAA-3' | < 1 |
| 3'-GCAGTMGGGCTTTT-5' | ||
| G24*/G27 | 5'-TTTTCGGGMTGACG-3' | < 1 |
| 3'-AAAAGMCMGACTGC-5' |