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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Protein Expr Purif. 2007 Nov 1;57(2):108–115. doi: 10.1016/j.pep.2007.10.018

Figure 1.

Figure 1

(a) Design of the GB1-fusion co-expression vector with two cloning and ribosomal binding sites (RBS). The coding sequences of the substrate protein and enzyme are respectively inserted into the gene1 and 2 cloning sites. The DNA sequence of linkers containing protease cleavage and cloning sites are listed. (b) Design of His6-tag (H6) GB1 fusion co-expression vector.