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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Protein Expr Purif. 2007 Nov 1;57(2):108–115. doi: 10.1016/j.pep.2007.10.018

Figure 2.

Figure 2

Expression of pKID and GB1pKID and thrombin-cleavage reaction of GB1pKID monitored by reversed-phase HPLC. (a) Comparison of expression levels between pKID and GB1pKID in E. coli cell lysates. Other E. coli proteins are indicated by asterisks (*). (b) Thrombin cleavage of GB1pKID using 1 unit/mg thrombin after 24-hour incubation at 25 °C. N-terminal Met of approximately 28% GB1 was truncated in E. coli cells.