FIGURE 5.

Differential expression of mitochondrial electron transport chain components in brain and RGC-5 cells. Isolated rat brain and RGC-5 mitochondria-enriched samples standardized for protein content by Bradford assay were compared to cytoplasmic samples for subunits of mitochondrial complexes I through IV and superoxide dismutase-2 (SOD-2). Samples were electrophoresed and transferred overnight to nitrocellulose, then incubated with appropriate primary and horseradish peroxidase-conjugated secondary antibodies. RGC-5 cells had less complex I through IV components and SOD-2 than brain, but the difference was greatest with respect to complex I and SOD-2, and to a lesser extent complex IV.