TABLE 2.

SUPEROXIDE PRODUCTION IN MITOCHONDRIA FROM BRAIN, UNDIFFERENTIATED RGC-5 CELLS, AND NEUROBLASTOMA SK-N-AS CELLS IN THE PRESENCE OF A COMPLEX II SUBSTRATE AND A COMPLEX III INHIBITOR^*

VARIABLE	BRAIN	UNDIFFERENTIATED RGC-5 CELLS	SK-N-AS NEUROBLASTOMA CELLS
	N = 7	N = 8	N = 7
Basal respiration	0.193 ± 0.027	0.028 ± 0.005	0.348 ± 0.042
Succinate	0.216 ± 0.022	0.031 ± 0.005	0.307 ± 0.024
Succinate + antimycin A	0.540 ± 0.116	0.024 ± 0.004	0.277 ± 0.034

^*The rates of superoxide production were determined after the addition of the complex II substrate succinate (10 mM) and the complex III inhibitor antimycin A (0.5 μM). Superoxide was indirectly measured using the H₂O₂ probe Amplex Red in the presence of horseradish peroxidase. The values are given in nmol H₂O₂/min/mg protein ± standard error of the mean, with N being the number of independent samples for each mitochondria type.