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. 2007 Nov 13;8(11):R240. doi: 10.1186/gb-2007-8-11-r240

Figure 1.

Figure 1

Validation of differentially expressed genes by RT-PCR in human ESCs, EBs and BCs. (a) Total RNA from human ESCs, EBs and BCs was used to construct cDNA pools, and the expression of genes was examined by semi-quantitative PCR. The number at the top of each lane indicates the amount (microliters) of cDNA used in the 50 μL PCR reaction. M = 100 bp DNA ladder. (b) Direct and (c) indirect analysis of differentially expressed genes matched the expression patterns obtained by RT-PCR. The fold change data are presented on the y-axis using logarithm-base-10.