Table 7.
Primer sequences
| Gene | Primer | Sequence | Annealing Tm (C) | MgCl2 conc. (μM) | Product size (bp) |
| HPRT | Sense Antisense |
5'-CTTGCGACCTTGACCATCTTTGGA-3' 5'-GGCGTCGTGATTAGTGATGATGAACC-3' |
58 | 1.5 | 467 |
| GATA-2 | Sense Antisense |
5'-TATGTGCCGGCGGCTGCCCACGACTACA-3' 5'-GGCTCTTCTGGCGGCCGACAGTCTT-3' |
60 | 1.5 | 280 |
| SCL | Sense Antisense |
5'-GAAGTGCTCCCCTCTGAAAGTT-3' 5'-GGCTATCTCTCCTCTGACCTCG-3' |
58 | 1.5 | 319 |
| β-Cluster globin genes | Sense Antisense |
5'-GTYTACCCHTGGACCCAGA-3' 5'-GCAGCTTGTCACAGTGCAG-3' |
56 | 2 | 190 |
| OCT4 | Sense Antisense |
5'-GAAGGTATTCAGCCAAACGAC-3' 5'-GTTACAGAACCACACTCGGA-3' |
55 | 2 | 315 |
| Nanog | Sense Antisense |
5'-TGCAAATGTCTTCTGCTGAGAT-3' 5'-GTTCAGGATGTTGGAGAGTTC-3' |
55 | 2 | 285 |
| Rex-1 | Sense Antisense |
5'-TGACAGGCAAGAAGCTTCCG-3' 5'-GCGTACGCAAATTAAAGTCCAGA-3' |
55 | 2 | 350 |