Figure 2. Effects of TMEV-infection on other spinal cord G-coupled protein receptors.

Quantitative real-time PCR was utilized to analyze spinal cord Sigma-1, CCR-1, CCR-5 and angiotensin II receptor type 1 (AT₁) mRNA expression in TMEV-infected and uninfected control mice. (A) TMEV infection induced a significant decrease in Sigma-1 receptor mRNA expression in both male and female mice at all time points tested (90, 150 and 180 days PI) compared to uninfected control mice (*p<0.0001). (B) TMEV infection induced a significant increase in chemokine CCR-1 receptor mRNA expression at all time points in both male and female mice at all time points tested compared to uninfected control mice (*p<0.0027). (C) TMEV infection induced a significant increase in chemokine CCR-5 receptor mRNA expression in both male and female mice at all time points tested compared to uninfected control mice (*p<0.0001). (D) TMEV infection did not alter AT₁ receptor mRNA expression in female mice at any of the time points tested. Moreover, AT₁ receptor mRNA expression was not altered in TMEV-treated male mice compared to uninfected controls until day 180 PI at which point there was a significant increase in expression (*p<0.0001). Results are displayed as the mean ± SEM (n = 5). Significant differences were determined using a two-way ANOVA followed by Bonferroni’s post hoc test.