Table 1.

Summary of the experimental design of the quantitative Real-Time PCR study indicating the numbers of mice per group, the total number of mice in the study (N) and the ANOVA F and p values.

Receptor	Number of mice/group	Number of conditions (time points, sex, TMEV vs control)	Total number of mice (N)	F Value	P value
MOR mRNA	4-5	12	52	14.677	<0.0001
DOR mRNA	4-5	12	51	13.687	<0.0001
KOR mRNA	4-5	12	51	9.859	<0.0001
Sigma-1 mRNA	4-5	12	53	15.251	<0.0001
CCR-1 mRNA	4-5	12	52	18.756	<0.0001
CCR-5 mRNA	4-5	12	52	11.272	<0.0001
AT1 mRNA	4-5	12	53	2.939	<0.0059

Note: Spinal cord mRNA from the same group of 53 mice was used for all Q-PCR experiments. The n varies among receptors due to lack of adequate amounts of cDNA from 2 mice.