Table 1.
S. cerevisiae yeast strains used in this study
| Strain | Genotype | Source |
|---|---|---|
| FF18733 | MATa, his7-2, leu2-3-112, lys1-1, trp1-289, ura3-52 | F. Fabre |
| FF18-1616 | FF18733 with rad27Δ::LEU2 | F. Fabre |
| DG100 | FF18733 with trf4Δ::kanMX6Myc | This study |
| DG200 | RAD27- with trf4Δ::kanMX6Myc | This study |
| CD186 | FF18733 with ogg1Δ::URA3, ntg1 ::LEU2, ntg2Δ::TRP1 | D. Thomas |
| DG300 | CD186 with trf4Δ::kanMX6Myc | This study |
| CY141 | MATa ade2-1, ura3-1, his3-11, 15 trp1-1, leu2-3 | M. Christman |
| CY143 | CY141 with 112 rDNA ::URA3 | M. Christman |
| CY1000 | CY143 with trf4Δ::HIS3 | M. Christman |
| DG400 | CY141 with pol4Δ::kanMX6Myc | This study |
| DG500 | CY1000 with pol4Δ::kanMX6Myc | This study |
| CY871 | CY143 with trf5Δ::LEU2 | M. Christman |
| DG101 | CY141 with trf4Δ(552–584)::kanMX6Myc | This study |
| DG102 | CY871 with trf4Δ(552–584)::kanMX6Myc | This study |
| Plasmid | Genotype | Source |
| pYX212 | URA3 2u | R&D Systems |
| pTRF4 | TRF4 URA3 2u | This study |
| pTRF4-552 | trf4-552 URA3 2u | This study |
| Yeplac112 | TRP1 2u | M. Christman |
| pSH4 | TRF5 TRP1 2u | M. Christman |