Figure 2.

Real-time PCR analysis of glomerular RNA from wild-type, Alport, DKO, and α1 integrin-deficient mice. Glomerular RNA from four independent sets of animals was analyzed for mRNA encoding the indicated MMPs. Data were normalized to GAPDH, which was run in multiplex with each sample. Differences in expression were significant if P < 0.05 (*, for comparison with wild-type mRNA levels; **, for comparison of Alport MMP-9 with α1^−/− or DKO). WT, wild type; Alp, Alport; DKO, α1 integrin-deficient Alport (double knockout); α1^−/−, α1 integrin-null.