Figure 3.

HD-5 AU-PAGE Western blot of small intestinal crypt epithelial cell extracts. Acid extracts of normal and active CD small intestinal crypts were separated by AU-PAGE, and after transfer to PVDF membrane, immunoblotting was performed using anti-HD-5 antibody. Electrophoretic mobility of HD-5 in crypt extracts from 10 samples affected by active CD (lanes 2 to 9, 11 to 12) was primarily similar to that for eight separate normal ileal crypt samples (lanes 1, 13 to 19) but distinct from that of synthetic mature HD-5 (lane 10). Lane 8 shows sample from resection specimen of patient CD6, whose ileostomy fluid was collected 5 weeks after operation for characterization of intraluminal HD-5 (see Table 2).