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. 2008 Mar;172(3):583–591. doi: 10.2353/ajpath.2008.070569

Figure 4.

Figure 4

Aged RAGE knockout (KO) mouse lungs spontaneously develop features of pulmonary fibrosis. Lung sections from 19- to 24-month-old wild-type (a, c, e) and RAGE KO (b, d, f) mice were subjected to immunohistochemical analysis for RAGE (a, b) and collagen type I (c, d) as well as Sirius Red staining for total collagen (e, f). RAGE knockout lungs lack immunoreactivity for RAGE (b) and show increased staining for collagen (d, f). g: Right lungs from 48-week-old wild-type (n = 3) or RAGE knockout mice (n = 4) were subjected to hydroxyproline analysis as an indicator of the extent of fibrosis. Forty-eight-week-old RAGE KO mice have significantly increased levels of hydroxyproline compared to age-matched wild-type controls (*P < 0.05).