Skip to main content
. 2008 Mar;172(3):738–747. doi: 10.2353/ajpath.2008.070097

Figure 7.

Figure 7

Effect of iron manipulation on insulin signaling in HepG2. A: Effect of a 24-hour treatment with FAC (150 μmol/L), Dfo (100 μmol/L), glucose (33 mmol/L, Gluc), and insulin (0.33 μmol/L, Ins) in the presence of FCS on Akt expression and activity, and on FoxO1 phosphorylation status. B: Effect of different concentrations of FAC and Dfo on Gsk3β expression and activity in cells cultured in the presence of FCS. C: Effect of Dfo on FoxO1-GFP intracellular localization. Results are representative of three independent experiments. Control, cells cultured without FCS for 8 hours; Dfo, cells preincubated with Dfo (100 μmol/L) for 24 hours. D: Effect of Dfo preincubation (100 μmol/L for 24 hours) on insulin induced (0.33 μmol/L for 15 minutes) Akt activation in cells cultured in the absence of FCS.