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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Neurobiol Dis. 2007 Sep 12;29(2):267–277. doi: 10.1016/j.nbd.2007.08.018

Fig. 6.

Fig. 6

Chronic disinhibition reduces levels of phosphorylated CREB (pCREB) in CA1 hippocampal pyramidal cells. (A) Low magnification confocal image of a hippocampal slice culture showing typical pattern of immunohistochemical staining for pCREB. (B and C) Comparison of pCREB staining in hippocampal area CA1 in a control slice culture (B) and a slice treated for 4 days with bicuculline (C). Notice the dramatic reduction in expression levels of pCREB. Also shown are the subpopulation of CA1 pyramidal cells that express YFP in YFP-H mice. (D-F) The nuclear localization of pCREB is YFP-positive CA1 pyramidal cells is clearly illustrated in merged images from a control slice. (G-I) Following 4 days of bicuculline treatment pCREB immunoreactivity in CA1 pyramidal cells remains nuclear but is greatly reduced in intensity. The majority of pCREB positive -YFP negative cells are CA1 pyramidal cells that do not express YFP. Arrow heads denote sites of co-localization. Scale bars: A = 500 μm. B and C = 100 μm. D - I = 20 μm.