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. Author manuscript; available in PMC: 2009 Feb 5.
Published in final edited form as: Virology. 2007 Nov 26;371(1):1–7. doi: 10.1016/j.virol.2007.10.021

Fig. 2.

Fig. 2

Fig. 2

(A) A portion of E1A proteins expressed in Ad2 infected HeLa cells is bound to chromatin. EtBr staining and E1A immunoblot analysis of chromatin-containing fractions from formaldehyde cross-linked Ad2 infected (30 moi) HeLa cells (24 h post-infection) after two rounds of isopycnic CsCl centrifugation. (B) E1A proteins associate with chromatin beginning at early times after Ad2 infection. Replicate flasks of HeLa cells were infected with 30 moi of Ad2. Cells were cross-linked with formaldehyde at the indicated times post-infection, chromatin isolated, and subjected to two rounds of isopycnic centrifugation on CsCl density gradients. Shown are E1A immunoblots of the density gradient fractions at different times after infection. Approximately equal amounts of chromatin from each time point were analyzed.