Skip to main content
. Author manuscript; available in PMC: 2009 Feb 5.
Published in final edited form as: Virology. 2007 Nov 26;371(1):1–7. doi: 10.1016/j.virol.2007.10.021

Fig. 4.

Fig. 4

Sequences within the E1A N-terminus are needed for the majority of E1A’s interaction with chromatin. Monolayer cultures of HeLa cells (T150) were infected with Ad/CMV/V5-DEST vectors (30 moi) expressing (i) full length E1A 243R, (ii) E1A 243R dl1101/1108/1135 containing deletions in the N-terminus, Rb binding site, and CtBP binding site, (iii) E1A 243R dl1101/1108 containing deletions in the N-terminus and the Rb binding site, and (iv) E1A 243R dl1108/1135 containing deletions in the Rb and CtBP binding sites. Cells were cross-linked with formaldehyde at 36 h post-infection, chromatin isolated, and subjected to CsCl density gradient centrifugation. Shown are E1A immunoblot analysis of the fractions from the various density gradients.