Figure 7.

TEV Cleavage of Rad21 in Cholinergic Neurons Induces Severe Locomotion Defects in Third-Instar Larvae

(A) Wandering third-instar larvae expressing TEV under the control of Cha-Gal4 and surviving on transgenic Rad21 with and without TEV sites were tested for motility (Rad21: Cha-Gal4/+; Rad21^ex3, Rad21-myc/Rad21^ex3, UAS-TEV; Rad21^TEV: Cha-Gal4/+; Rad21^ex15, Rad21^TEV/Rad21^ex3, UAS-TEV). Larval movements were tracked and superimposed to a grid. Locomotion was measured by the number of grid squares each larva traveled through. The number of larvae that traveled through the indicated number of squares (1–5, 6–10, etc.) is shown as a percentage of the total number of larvae tested (54 and 48 for strains containing Rad21 and Rad21^TEV, respectively).

(B) Representative images and temporal projections of movements from larvae that express TEV in cholinergic neurons and survive on either transgenic Rad21 (i and i′) or Rad21^TEV (ii–v′) (same genotypes as in [A]). (i)–(v) show the initial position of the larvae. H indicates the position of the head. (i′)–(v′) show the temporal projections of the images taken over a 20 s interval (images taken every 2 s). Note that controls move mostly straight, whereas larvae in which Rad21^TEV has been cleaved in cholinergic neurons show frequent episodes of turns, head movement, and backward motion.