Table 1.

Effect of Succinate on the Interaction of Complex I with NADH-OH

	second-order rate constant kon × 10−8 (M−1 min−1)
	− succinate	+ succinate (5 mM)
	1.7 + 0.4a	0.15 + 0.02a
	1.5 + 0.4b
+ rotenone (5 μM)		1.5 + 0.2c
+ antimycin A (1 μg/mL)		1.3 + 0.2d
+ gramicidin D (0.05 μg/mL)		1.3 + 0.2d
first-order rate constant koff (min−1)	0.05d	0.09d

^aDetermined as in Figure 1.

^bDetermined as in Figure 1, except that residual NADH:HAR reductase activity was assayed in the presence of 100 μM NADH, 0.5 mM HAR, and 5 μM rotenone.

^cDetermined as in Figure 1; the inhibitors were present at the concentration indicated.

^dDetermined as in Figure 4.