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. 2008 Mar 12;3(3):e1773. doi: 10.1371/journal.pone.0001773

Figure 1. 2 % agarose gel, 16S rRNA PCR of gDNA extracted from archival blood samples 1 to 6.

Figure 1

2 µl of gDNA in 25 µl PCR reaction with 25×PCR cycles. 5 µl loaded on gel. Image is reverse colour under UV transillumination (Bio-Rad). Positive control (+Control) used 2 µl of gDNA from a local Streptococcus pneumoniae isolate. Non template control (NTC) consisting of 25 µl PCR mix only, water control 2 µl molecular grade water in place of gDNA.